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Overview
Analytical Media for HPLC and UPLC refers to high-purity stationary phases, typically silica-based or polymer-coated particles, engineered for ultra-high pressure chromatography. These media feature precise particle size distributions (1.7–5 µm) and rigorous surface chemistry control to ensure maximum efficiency in separating complex mixtures.
Application
- Pharmaceuticals: API purity testing in Agilent and Waters systems.
- Petrochemicals: Hydrocarbon analysis using Thermo Fisher columns.
- Environmental: Pesticide residue detection in Shimadzu UPLC units.
- Food Safety: Additive quantification in standard ISO 17025 labs.
Precautions
- Operating Pressure: Maintain below 15,000 psi for UPLC; 6,000 psi for HPLC.
- Environment: Store at 15–25°C with humidity under 60% RH.
- Maintenance: Flush columns with 100% organic solvent post-run to prevent salt precipitation.