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Dioxygenases (DO) are a class of enzymes catalyzing the incorporation of both atoms of molecular oxygen into organic substrates. These biocatalysts typically require iron or copper cofactors and operate via oxidative mechanisms essential for metabolic pathways. Specific variants like 2-oxoglutarate-dependent dioxygenase utilize 2-OG as a co-substrate. Molecular formula and CAS numbers vary by specific isoform; general classes lack a single unified identifier.
Strict anaerobic handling is often required during storage to prevent enzyme denaturation. Personnel must wear appropriate PPE including gloves and goggles due to potential allergenicity. Storage conditions typically mandate temperatures below -20°C with lyophilized forms preferred for long-term stability.