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*The following content is generated by AI and is for reference only.
Overview
Geneticin (G418) is an aminoglycoside antibiotic used primarily as a selection agent in molecular biology. It inhibits protein synthesis by binding to the 80S ribosome. The compound features the molecular formula C23H43N7O9S and CAS No. 80604-65-5.
Application
- Mammalian Cell Culture: Selection of stable transfectants expressing neomycin resistance genes.
- Viral Vector Production: Maintenance of cell lines producing recombinant lentivirus or retrovirus.
- Research Tools: Screening for gene integration efficiency in CRISPR-Cas9 workflows.
Precautions
Strictly for research use only; not approved for human clinical therapy. Store powder at -20°C protected from light. Handle with appropriate PPE due to potential mutagenic properties.