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Overview
Hydrophobic Interaction Media consists of **hydrophobic ligands** covalently attached to a rigid matrix, enabling protein purification via salt-induced adsorption. The core material utilizes cross-linked agarose or polymers with alkyl groups.
Application
- Biopharmaceuticals: Monoclonal antibodies in chromatography systems.
- Biochemicals: Enzyme isolation in simulated moving bed reactors.
- Fermentation: Downstream processing in stirred-tank bioreactors.
Precautions
- Environment: Maintain 4-25°C; relative humidity below 60%.
- Pressure: Operate within 0.1-0.5 MPa to prevent column compaction.
- Maintenance: Perform weekly CIP with NaOH; store in 20% ethanol.