Category:
Other Specialized Reagents
CAS NO:
68399-78-0
Standard:
USP,
N-(Hydroxyethyl)piperazine-N-2-hydroxypropanesulfonic acid, commonly known as HEPES, is a zwitterionic buffering agent synthesized via the reaction of piperazine with 2-hydroxypropane-1-sulfonic acid. Its molecular formula is C8H18N2O4S and CAS number is 7365-45-9. This compound maintains stable pH in biological systems across a broad range.
While generally safe for laboratory use, HEPES can generate reactive oxygen species under light exposure; store in opaque containers at controlled temperatures. Avoid direct contact with skin and eyes. Not approved for systemic human administration in high concentrations due to potential cytotoxicity.