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Overview
Trimethyl Phosphate-d9 is a fully deuterated isotopologue of trimethyl phosphate, primarily synthesized via the esterification of phosphoric acid with deuterated methanol. It serves as a critical internal standard in mass spectrometry and NMR spectroscopy for quantitative analysis.
- Molecular Formula: (CD3)3PO4
- CAS Number: 1786-26-5
Application
- Analytical Chemistry: Used as an internal standard to correct matrix effects during LC-MS/MS quantification of organophosphorus compounds.
- Metabolic Research: Employed in stable isotope labeling studies to trace metabolic pathways without altering chemical reactivity significantly.
- Spectroscopy: Provides a reference signal in 31P-NMR to calibrate chemical shifts in complex solvent systems.
Precautions
- Storage: Must be stored under inert atmosphere at temperatures below -20°C to prevent hydrolysis and moisture absorption.
- Safety: Handle in a fume hood; avoid skin contact as it may cause irritation and is toxic if ingested.
- Regulatory: Not approved for direct clinical use; restricted to laboratory research applications only.